The membrane strip comprises a universal primer, a solid phase carrier, a probe and a PCR positive quality control plasmid, wherein the probe is fixed on the carrier the universal primer is a degenerate primer pair TF/TR the solid phase carrier is a Biodyne C membrane the probe fixed on the carrier is a group of arrays consisting of a quality control probe and an RDB detection probe the quality control probe comprises a color development point probe, a positive comparison probe and a negative comparison probe and the RDB detection probe is a section of 15 to 19nt oligonucleotide of which the 5' end is marked with amino (-NH2). The invention relates to a reverse dot blot (RDB) detection membrane strip, a detection method and a kit for identifying insects. Application filed by Shenzhen Academy of Inspection and Quarantine, Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry Exit Inspection and Quarantine Bureau filed Critical Shenzhen Academy of Inspection and Quarantine Priority to CN201010228487.0A priority Critical patent/CN101914617B/en Publication of CN101914617A publication Critical patent/CN101914617A/en Application granted granted Critical Publication of CN101914617B publication Critical patent/CN101914617B/en Status Expired - Fee Related legal-status Critical Current Anticipated expiration legal-status Critical Links Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Family has litigation First worldwide family litigation filed litigation Critical (A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License. Original Assignee Shenzhen Academy of Inspection and Quarantine Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry Exit Inspection and Quarantine Bureau Priority date (The priority date is an assumption and is not a legal conclusion. Shenzhen Academy of Inspection and QuarantineĪnimal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry Exit Inspection and Quarantine Bureau Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ( en Inventor 余道坚 徐浪 焦懿 陈志粦 郑耘 康林 王银竹 陈乃中 徐孟杰 胡斌 Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number CN2010102284870A Other languages Chinese ( zh) Google Patents CN101914617A - Reverse dot blot (RDB) detection membrane strip, detection method and kit for identifying insects CN101914617A - Reverse dot blot (RDB) detection membrane strip, detection method and kit for identifying insects
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